Figure 3.

Assembly and release of Moloney virions measured by reverse transcriptase (RT) assay. (A) Spots indicate the amount of labeled nucleotide incorporated in an exogenous RT assay on a homopolymer template by virion-associated RT. Equal amounts of virus were used to infect naïve Rat2, the N-Msn-zeo line isolated from the library screen (RL34), the stably expressing zeocin-tagged C-terminally truncated (N-Msn-zeo:7) or the full-length (Msn-zeo:3) moesin lines. Culture supernatants were collected on successive days as indicated, and virus production was monitored by RT assay. Mock, no virus was applied. (B) Quantification of viral replication assay shown in (A). Dot intensities were normalized against uninfected cell samples.