Figure 8.

RNAi-mediated knockdown of moesin increases the levels of stable microtubule formation in both wt Rat2 cells and the mutant R4-7 line. (A) The wt Rat2 cells or mutant R4-7 cells were transfected with two independent short-interfering RNAs (indicated as Msn-A, Msn-B) on two consecutive days with equal amounts of specific RNA duplexes or a nonspecific GFP duplex. The cells were subsequently fixed and stained with a rabbit polyclonal antibody for stable Glu-MTs (A) and a rat mAb for dynamic Tyr-MTs (B). The cells were then incubated with fluorescently labeled secondary antibodies and images were taken as described in Materials and methods.