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. 2006 Dec 7;26(1):170–183. doi: 10.1038/sj.emboj.7601463

Figure 4.

Figure 4

PCR analysis of DSB repair in lig-4/cku-80-defective backgrounds. (A) Details of the experimental procedure used to study DSB repair by PCR. unc-5(ox171∷Mos1) adults were heat-shocked (t=0) to induce Mos1 excision and DSB repair was analyzed by PCR with primers flanking the Mos1 insertion point (t=18 h). PCR products were analyzed on an agarose gel: (−) non-heat-shocked, (+) heat-shocked samples, M=size marker (1 kb Plus DNA Ladder, Invitrogen). (B) Sequence analysis of footprints generated in wild-type and cku-80 and lig-4 mutants. See Table I footnotes for legends. Numbers in parentheses represent the numbers of footprints analyzed per genotype.