Figure 5.
Oligomerization of EpsE/EpsL(1–253) in the presence of cardiolipin and ATP. Reactions containing EpsE/EpsL(1–253) or EpsE/EpsL(1–242) with cardiolipin, ATP and MgCl2 were crosslinked with the addition of increasing concentrations of DSP (0, 10, 50, 100, and 200 μM). Reactions were analyzed by SDS–PAGE and immunoblotting with antibodies to EpsE or EpsL. The positions of monomeric forms of EpsE and cyto-EpsL, as well as molecular mass markers are indicated. EpsL* marks the position of cyto-EpsL, either EpsL(1–253) or EpsL(1–242) where indicated.