Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
C-terminal tail point mutants can function in p53 degradation if oligomerized with wild-type MDM2. (A) U2OS cells were transiently transfected with FLAG-p53, GFP and different ratios of wild-type MDM2 to Y489A or Y489D mutants (to give a constant total amount of transfected MDM2 plasmid of 1.6 μg) and analyzed by Western blotting. (B) FLAG-p53 was transiently cotransfected into U2OS cells with wild-type MDM2 and C-terminal tail mutants in a 1:1 ratio.
