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. 2006 Dec 14;26(1):102–112. doi: 10.1038/sj.emboj.7601469

Figure 9.

Figure 9

(A) Reactivation of MDM2 C-terminal tail mutants by MDMX requires intact MDMX C-terminal tail. U2OS cells were transfected with MDM2 Y489A mutants and wild-type Myc-MDMX or MDMX C-terminal tail mutants in a 1:1 ratio, together with FLAG-p53 and GFP and analyzed by Western blotting. (B) Interaction with MDMX C-terminal tail point mutant F488A inhibits MDM2 function. The experiment was performed using wild-type MDM2 in the way described in (A) for Y489A mutant. (C) C-terminal tail sequences of MDM2, MDMX, neuralized and IAP proteins were aligned using BOXSHADE 3.21 at http://www.ch.embnet.org/software/BOX_form.html. (D) Y489 can be substituted for other aromatic amino acids. U2OS cells were transiently cotransfected with FLAG-p53 and MDM2 Y489A, Y489F and Y489H point mutants and analyzed by Western blotting.