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. 2006 Dec 14;26(1):65–75. doi: 10.1038/sj.emboj.7601483

Figure 4.

Figure 4

Casp8 disrupts the AR N/C interaction. (A) The mammalian two-hybrid assay was performed in PC3mm2 cells. Cells were transfected with pACT-AR/N, pBIND-AR/C, or both. Cells were transfected with pACT-ID or pBIND-MyoD as control. Transfected cells were grown in the presence of 10 nM R1881 for 48 h and then harvested for luciferase assay. Casp8 Expression was detected by RT–PCR (bottom panel). (B) An intact AR is required for Casp8-mediated inhibition of AR transactivation. pGL3-4 × ARE-E4-luc, pcDNA-AR, pcDNA-ARΔC271, or pcDNA-ARΔN206 and pcDNA-Casp8 were cotransfected into PC3mm2 cells. Cells were grown in the presence of 10 nM R1881 for 48 h and then harvested for luciferase assay. Values represent mean±s.d. (N=3).