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. 2006 Dec 14;26(1):65–75. doi: 10.1038/sj.emboj.7601483

Figure 9.

Figure 9

(A) Casp8 inhibits AR interaction with DNA in LNCaP cells. NE were prepared from LNCaP (lanes 2, 3, 8 and 9) or LNCaP trasfected with pcDNA-Casp8 (lanes 4 and 10), retrovirus vector (lanes 5 and 11), or Casp8 shRNA (#3) (lanes 6 and 12). (B) Casp8 expression inhibited the androgen-driven AR nuclear localization. LNCaP cells or LNCaP cells transfected with pcDNA-EGFP-Casp8 were grown in the absence (panels a–d, i, j) or presence (panels e–h, k, l) of 10 nM R1881. Signals were detected for nucleus (blue) (panels a, e, i, k), AR (red) (panels b, f, j, l), and EGFP-Casp8 (Green) (panels c, g) under a confocal microscope. In panel f, EGFP-Casp8-positive and -negative cells are indicated by white and blue arrows, respectively. Panels d and h are merged images of panels b and c and f and g, respectively.