Figure 3.

Treatment of T cells or APC with CT has the same effect on IFN-γ and IL-5 production and proliferation. LNC from mice immunized with type II collagen in CFA were separated into CD4⁺ T-cell and APC fractions by positive selection of CD4⁺ T cells, followed by gamma-irradiation of the APC fraction. The T-cell and APC populations were treated separately with CT for 1 hr at 37°, washed and then recombined, as shown. The recombined cells were cultured in the presence of type II collagen (50 μg/ml) for 72 hr for measurement of IFN-γ and IL-5 production. To measure proliferation, [³H]thymidine was added for the last 16 hr. Data are shown as mean ± SE and the experiment is representative of three experiments, all of which gave the same result. P-values refer to differences between CT-treated groups and untreated controls.