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. 2004 Jan;111(1):75–85. doi: 10.1111/j.1365-2567.2003.01773.x

Figure 2.

Figure 2

Effect of NF-κB inhibitors on cytokine-dependent increases in pIgR protein measured by Western blot analysis. HT29 cells were preincubated without and with the indicated concentration of inhibitor for 1 hr. The indicated cytokines were then added and cultures were incubated for 30 hr at 37°: IL-4, 10 ng/ml; IFN-γ, 200 U/ml; or both together (IL-4/IFN-γ). At the end of the incubation period, whole cell extracts were prepared and pIgR protein was determined by Western blot analysis using a monoclonal antibody against human secretory component. The 98 000 MW band represents the full-sized receptor and the 78 000 MW band represents secretory component. Similar results were seen in three or more independent experiments for each condition.