Figure 5.

Effect of overexpressing the IκBα-serine mutant on induction of IRF-1. HT29 cells were transfected with 100 MOI of an adenoviral vector expressing β -galactosidase (LacZ, controls for adenoviral effects) or IκBα-serine mutant (IκB) overnight. Medium without (Cont) or with IL-4 (10 ng/ml) or IFN-γ (200 U/ml) was then added and cultures were incubated for 24 hr at 37°. Whole cell extracts were prepared and IRF-1 protein was measured by Western blot analysis (upper panel). Blots were stripped and reblotted with antibody against IκBα (lower panel).