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. 2004 Jan;111(1):75–85. doi: 10.1111/j.1365-2567.2003.01773.x

Figure 6.

Figure 6

Effect of adding NF-κB inhibitor at varying times on cytokine-dependent increases in pIgR protein. HT29 cells were incubated without (Cont) or with IL-4 (10 ng/ml) and IFN-γ (200 U/ml) together. MG115 was added at the indicated time relative to cytokine addition (0 hr). All cultures were harvested at the same time (48 hr) after cytokine addition. Whole cell extracts were prepared and pIgR protein was measured by Western blot analysis using a monoclonal antibody against human secretory component. The 98 000 MW band represents the full-sized receptor and the 78 000 MW band represents secretory component.