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. 2004 Jan;111(1):75–85. doi: 10.1111/j.1365-2567.2003.01773.x

Figure 9.

Figure 9

Effect of over-expressing the IκBα-serine mutant on cytokine-dependent reporter gene expression. HT29 cells were cotransfected with the indicated plasmid and 100 MOI of an empty adenoviral vector or one expressing the IκBα-serine mutant overnight. Cultures were then treated with the indicated cytokine for 48 hr: IL-4, TNF-α, and IL-1β, each 10 ng/ml; IFN-γ, 200 U/ml. SEAP activity was measured in the medium. The percentage SEAP activity remaining in medium from cells transfected with the IκBα-serine mutant relative to cells transfected with empty adenovirus was determined. Values represent the mean ± SEM (n ≥ 12) for combined data from three or more independent experiments with quadruplicate samples per experiment. A significant decrease was observed with the IκBα-serine mutant relative to the empty vector control; *P < 0.001, **P < 0.05. †No cytokine-dependent increases in SEAP expression under these conditions.