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Production of nitric oxide by Mφ and DC exposed to TLR ligands. Purified bovine Mφ or DC were exposed to various TLR ligands or whole bacteria, either alone or with 100 U of rboIFN-γ per ml (pattern bars). Cells treated with medium served as negative controls. levels were determined by the Griess assay. The results are presented as the mean NO concentrations for triplicate cultures and 1 SD, and show representative data from one animal. S.d. = S. dublin; L.m. = L. monocytogenes.
