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Effect of TNF-α treatment on the migration of neutrophils towards hBD-2. Neutrophils treated with increasing concentrations of TNF-α for 6 hr were placed into the upper wells of the microchamber and allowed to migrate towards 1–10 µg/ml hBD-2, 4 µg/ml MIP-3α and 10−8 m fMLP for 1 hr at 37°. Neutrophil chemotaxis was assessed by counting the number of migrated cells through the polycarbonate membrane. Each bar represents the mean of seven separate experiments.
