Table 1.
Checkerboard analysis of hBD-2-induced neutrophil migration
| (a) | |||||
|---|---|---|---|---|---|
| hBD-2 in upper chamber (mg/ml) | |||||
| hBD-2 in lower chamber (mg/ml) | 0 | 1 | 2·5 | 5 | 10 |
| 0 | 41 ± 6 | 32 ± 5 | 39 ± 9 | 51 ± 4 | 26 ± 7 |
| 1 | 76 ± 4 | 44 ± 2 | 26 ± 4 | 39 ± 8 | 61 ± 9 |
| 2·5 | 169 ± 33 | 107 ± 27 | 76 ± 11 | 38 ± 6 | 53 ± 4 |
| 5 | 286 ± 62 | 223 ± 39 | 157 ± 17 | 114 ± 23 | 59 ± 5 |
| 10 | 158 ± 17 | 99 ± 16 | 73 ± 11 | 82 ± 8 | 79 ± 7 |
| (b) | |||||
|---|---|---|---|---|---|
| MIP-3α in upper chamber (μg/ml) | |||||
| hBD-2 in lower chamber (μg/ml) | 0 | 1 | 2 | 4 | 8 |
| 0 | 41 ± 6 | 52 ± 2 | 37 ± 7 | 53 ± 5 | 36 ± 7 |
| 1 | 76 ± 4 | 73 ± 9 | 46 ± 6 | 37 ± 9 | 44 ± 5 |
| 2·5 | 169 ± 33 | 152 ± 26 | 109 ± 21 | 68 ± 12 | 69 ± 5 |
| 5 | 286 ± 62 | 263 ± 43 | 188 ± 37 | 103 ± 8 | 67 ± 5 |
| 10 | 158 ± 17 | 134 ± 17 | 99 ± 25 | 70 ± 6 | 82 ± 3 |
Various concentrations of hBD-2 were added to the lower chambers, and TNF-α-treated neutrophils in the upper chambers containing either hBD-2 (a) or MIP-3α (b) at indicated concentrations were allowed to migrate. The migratory response was measured after a 60 min-incubation by counting the number of cells through the polycarbonate membrane. Each value represents the mean ± SD of three separate experiments.