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Fractionation of purified TBA61: (a) Protein elution profile from Superdex-200 gel column. Eluates were pooled to fractions I and II (excluding the overlapping region) corresponding to polymers and monomer of IgA. (b) Analysis by 4–12% gradient SDS–PAGE and silver staining; 1. Unfractionated IgA, 2. monomer (fraction II), 3. Polymers (fraction I). R. reduced unfractionated TBA61. Heavy and light immunoglobulin chains are indicated.
