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. 2004 Mar;111(3):323–327. doi: 10.1111/j.1365-2567.2004.01812.x

Figure 2.

Figure 2

(a) Secretion of interferon-γ (IFN-γ) by cultures of spleen cells from mice infected with Mycobacterium avium strain 2447 or strain 1983 upon stimulation with homologous M. avium envelope proteins (4 µg/ml). Levels of IFN-γ in non-stimulated cultures, or in stimulated cultures of spleen cells from non-infected animals, were ≈500 pg/ml. (b) Measurement, by semiquantitative reverse transcription–polymerase chain reaction (RT–PCR), of interleukin-12 p40 (IL-12p40) expression in liver samples of mice infected for 15 or 30 days with M. avium 2447. Each band represents the digital image of the amplification product for the material obtained for one mouse and for either IL-12p40 or hypoxanthine phosphorybosyltransferase (HPRT).