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. 2004 Apr;111(4):407–415. doi: 10.1111/j.1365-2567.2004.01822.x

Figure 4.

Figure 4

Src protein tyrosine kinase (PTK) activation is involved in interleukin-8 (CXCL8)-mediated CXCL8 chemotaxis. (a) Human polymorphonuclear neutrophils (PMNs) were preincubated with vehicle or PP1 (3 µm, 10 µm or 30 µm) for 15 min at 37°. (b) PMNs were preincubated with neutralizing anti-CXCR2 immunoglobulin (3 µg/ml), in the presence or absence of PP1 (3 µm or 10 µm), for 15 min at 37°. (c) PMNs were preincubated with neutralizing anti-CXCR1 (3 µg/ml) immunoglobulin, in the presence or absence of PP1 (3 µm or 10 µm), for 15 min at 37°. Cells were then tested for their ability to migrate in response to CXCL8 (10 ng/ml). Data are expressed as mean values ± standard deviation (SD) of three independent experiments. Spontaneous PMN migration was 13 ± 4. *P < 0·05 versus CXCL8 alone (Mann–Whitney U-test); **P < 0·01 versus CXCL8 alone (Mann–Whitney U-test); ‡P < 0·05 versus anti-CXCR2 alone pretreated group (Mann–Whitney U-test).