Figure 7.

Src protein tyrosine kinase (PTK) activation is involved in interleukin-8 (CXCL8)-induced HL-60-derived PMN-like cell migration. HL-60-derived human polymorphonuclear neutrophil (PMN)-like cells were transiently transfected with pcDNA-derived plasmid expressing PykM, or with empty pcDNA3. Cells were preincubated with vehicle or PP1 (10 µm) for 15 min at 37° and then tested for their ability to migrate in response to CXCL8 (100 ng/ml). Data are expressed as mean values ± standard deviation (SD) of three independent experiments. **P < 0·01 versus the corresponding vehicle-treated group (Mann–Whitney U-test).