Figure 4.

Purified SIC fragments. (a) Tricine-SDS gel of purified SIC and fragments. (b) ELISA—detection of SIC and fragments by rabbit anti-SIC. Analysis of purified elastase-digested fragments of SIC. (a) SIC and purified elastase-digested fragments of SIC were run on a 16% Tricine SDS-PAGE gel and Coomassie stained. Lane 1, molecular weight markers; lane 2, fragment A, amino acids 1–33; lane 3, fragment B, amino acids 34–126; lane 4, fragment C, amino acids 127–273; lane 5, whole SIC. (b) An ELISA plate was coated with SIC or purified elastase-digested fragments of SIC, and bound protein detected. Bar 1 = intact SIC, bar 2 = fragment A, bar 3 = fragment B, bar 4 = fragment C. Results are expressed as net OD₄₀₅ after deduction of background values from matching uncoated wells, ± SEM. Where no error bars are shown, the SEM is too small to be seen. Controls of coated wells plus second antibody are not shown, but all values were < 0·07.