Table 2.
T-cell responses to the corresponding peptides of ML and MT GroES proteins; results are given as percentage of donors giving a positive signal (cut-off shown in the table below each parameter)
| Immune parameters | L | T | EC | P | L | T | EC | P | L | T | EC | P | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LTT | LTT | LTT | LTT | IFN-γ | IFN-γ | IFN-γ | IFN-γ | IL-10 | IL-10 | IL-10 | IL-10 | ||
| Amino acid no. | Cut-off values for positivity Amino acid sequence | 2 SI > 1500 δ cpm | >χ + 2 SD > δ 20 pg/ml | >χ + 2 SD > δ 20 pg/ml | |||||||||
| L1-15 | VAKVKIKPLEDKILV (ML) | 22 | |||||||||||
| T1-15 | -----N--------- (MT) | 11 | |||||||||||
| L11-25 | DKILVQAGEAETMTP (ML) | 11 | 10 | 25 | 20 | 25 | 11 | ||||||
| T11-25 | -------N----T-A (MT) | 11 | 10 | 25 | 30 | 25 | 11 | ||||||
| L21-35 | ETMTPSGLVIPENAK (ML) | 10 | 11 | 11 | 11 | 20 | 25 | ||||||
| T21-35 | --T-A------DT-- (MT) | 10 | 11 | ||||||||||
| L31-45 | PENAKEKPQEGTVVA (ML) | 11 | 10 | ||||||||||
| T31-45 | -DT------------ (MT) | 11 | 11 | 22 | |||||||||
| L51-65 | WDEDGAKRIPVDVSE (ML) | 10 | 11 | ||||||||||
| T51-65 | ---------E-----L-A- (MT) | 11 | 10 | 11 | 11 | 10 | 22 | ||||||
| L61-75 | VDVSEGDIVIYSKYG (ML) | 38 | 11 | 25 | 11 | 11 | 10 | ||||||
| T61-75 | L-A----T------- (MT) | 13 | 11 | 10 | 25 | 11 | 11 | 11 | |||||
| ML10K | >M. leprae | 33 | 40 | 50 | 11 | 44 | 50 | 63 | 33 | 78 | 90 | 88 | 67 |
| MT10K | M. tuberculosis | 22 | 30 | 63 | 11 | 33 | 60 | 50 | 11 | 67 | 70 | 13 | 79 |
Italic data indicate concurrent recognition of ML and MT GroES peptides. Bold data indicate that responses were present only in leprosy patients to ML peptide only. All other details including the donors in each group are as described in Fig. 2.