Table 3.
Cytokine profiles induced in peripheral blood mononuclear cells (PBMCs), and in CD4+, CD4−, CD14+ and CD14− subpopulations, in response to different stimulations
| Medium | 2006 | 2216 | LPS | pIC | % Purity | |
|---|---|---|---|---|---|---|
| IFN-α (U/ml) | ||||||
| PBMC | <3 | <3 | 6325 ± 370 | <3 | <3 | |
| CD4+ | <3 | <3 | 20 070 ± 1840 | <3 | <3 | 87 |
| CD4− | <3 | <3 | 1505 ± 230 | <3 | <3 | 6 |
| CD14+ | <3 | <3 | 2290 ± 20 | <3 | <3 | 96 |
| CD14− | <3 | <3 | 23 940 ± 650 | <3 | <3 | 1 |
| TNF-α (pg/ml) | ||||||
| PBMC | <5 | <5 | 419 ± 44 | ND | ND | |
| CD4+ | <5 | <5 | 137 ± 33 | ND | ND | 87 |
| CD4− | <5 | <5 | 97 ± 21 | ND | ND | 6 |
| CD14+ | <5 | <5 | 12 ± 6 | ND | ND | 96 |
| CD14− | <5 | <5 | 236 ± 22 | ND | ND | 1 |
| IL-10 (pg/ml) | ||||||
| CD4+ | <8 | <8 | <8 | <8 | <8 | 87 |
| CD4− | <8 | <8 | <8 | 473 ± 11 | 382 ± 4 | 6 |
| CD14+ | <8 | <8 | <8 | 469 ± 10 | 198 ± 3 | 96 |
| CD14− | <8 | <8 | <8 | 371 ± 32 | 405 ± 23 | 1 |
| IL-12 (pg/ml) | ||||||
| CD4+ | <15 | <15 | 191 ± 18 | <15 | <15 | 87 |
| CD4− | <15 | <15 | <15 | <15 | <15 | 6 |
| CD14+ | <15 | <15 | <15 | <15 | <15 | 96 |
| CD14− | <15 | <15 | 30 ± 4 | <15 | <15 | 1 |
PBMCs were separated into CD4 and CD14 fractions by magnetic-activated cell (MACS) sorting. Fractions were then stimulated with the CpG-ODNs 2006 or 2216 (10 µg/ml), lipopolysaccharide (LPS) (1 µg/ml) or Poly I : C (pIC) (10 µg/ml). The cell supernatants were harvested after 24 hr and tested, by enzyme-linked immunosorbent assay (ELISA), for the presence of interferon-α (IFN-α), tumour necrosis factor-α(TNF-α), interleukin (IL)-10 and IL-12. One representative experiment out of two is shown. ND, not determined.