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. 2004 May;112(1):105–116. doi: 10.1111/j.1365-2567.2004.01853.x

Figure 3.

Figure 3

Long-term expansion of murine lymphocytes. The culture conditions for the indicated combinations of purified T cells, aAPCs, and beads were as in Fig. 2. CD137L-expressing and non-CD137L-expressing aAPC were loaded with anti-CD3 and anti-CD28 (K-CD3/28, K137L-CD3/28, Y-CD3/28, or Y137L-CD3/28). (a) For CD8 expansion, K137L-CD3/28 (solid triangles) and Y137L-CD3/28 (solid circles) cocultures were stimulated on days 0, 8, and 16, while CD3/28 beads (open squares), K-CD3/28 (open triangles), and Y-CD3/28 (open circles) were stimulated on days 0 and 8 only. (b) For CD4 expansion, K137L-CD3/28 cocultures were stimulated on days 0, 10, 19, and 27, while all others were stimulated on days 0 and 10. Data is representative of three separate expansion experiments.