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. 2004 May;112(1):105–116. doi: 10.1111/j.1365-2567.2004.01853.x

Figure 5.

Figure 5

Analysis of memory cell markers on naïve, immunized, and aAPC expanded CD8+-splenocytes. Expression of CD62L (a, d, g), CD44 (c, f, i), and CD69 (b, e, h) was analysed by three-colour flow cytometric analysis of CyChrome-CD8-gated splenocytes from naïve mice (a, b, c), naïve splenocytes cultured for 8 days with K562/CD137L aAPC loaded with anti-CD3 and anti-CD28 (g, h, i), or splenocytes isolated directly from AGN2a-CD80/CD137L immunized A/J mice. Two-colour flow cytometric profiles for DX5 and CD62L (a, d, g), CD69 (b, e, h), or CD44 (c, f, i) expression are shown.