Table 2.

2C3–Ig sequence analysis by prediction software (syfpeithi and bimas programs) to identify cytotoxic T-lymphocyte (CTL) epitopes from associated complementarity-determining regions (CDRs) and framework regions (FRs)

			MHC binding score
			syfpeithi22			bimas23
Origin	Position	Sequence	Kd	Ld	Kd	Dd	Ld
IgH	CDR3	YYGSSYGFA	20	13	72	2	13
IgH	FR1 + CDR1	GFTFSDYYI	18	9	960	2·88	10
IgH	FR1	RLVESGGGL	17	11	115·2	1·2	5
IgH	FR1	GLVKPGGSL	16	5	80	1	5
IgH	CDR1	IYWVRQTPE	16	3	100	<1	<1
IgH	CDR3	GYYGSSYGF	13	12	60	<1	10
IgH	CDR2	SDGGSYTYY	12	3	<1	<1	<1
IgH	CDR1	TFSDYYIYW	10	8	24	<1	<1
IgKappa	FR1	TPLSLPVSL	20	21	115·2	<1	150
IgKappa	CDR3	YFCSQSTHV	19	<1	288·0	<1	<1
IgKappa	CDR3 + FR4	WTFGGGTKL	18	13	57·6	<1	6·5
IgKappa	FR1	VSLGDQASI	17	14	96	<1	<1
IgKappa	FR1	SCRSSQSLV	17	<1	57·6	<1	<1
IgKappa	FR3	SGSGTDFTL	16	12	48	12	<1
IgKappa	FR1	ISCRSSQSL	15	20	48	<1	25
IgKappa	CDR1	VHSNGNTYL	15	15	<1	<1	10
IgKappa	CDR2	IYKVSNRFS	15	<1	60	<1	10
IgKappa	CDR3 + FR4	TFGGGTKLE	13	3	<1	<1	6·5

MHC, major histocompatibility complex.