Table 1.

Binding of tgD and tgD-CD154 to blood leucocyte population^*

	% protein binding†
	tgD	tgD-CD154
T cells	0·5	0·7
B cells	0·0	46·5
Monocytes	1·2	11·6
Total	1·7	58·8

^*Dual labelling was performed by first incubating PBMCs with medium or transfected culture supernatants containing tgD or tgD-CD154, and then with a mixture of gD-specific mAb 1G6 and antibodies to various cell surface molecules. Isotype-specific goat anti-mouse immunoglobulin conjugated to either FITC or PE was used to detect anti-gD and anti-leucocyte mAbs.

^†Calculated based on the formula cells positive for tgD + cell marker/cells positive for cell marker.

Values obtained after subtracting autofluorescence following incubation with medium alone.