Figure 2.
(a) Constitutive secretion of CCL19-IgG2b does not suppress J558 tumour growth in syngeneic BALB/c mice. 5 × 106 cells/200 μl PBS were injected s.c. in female 6–8-week-old BALB/c mice. The size of tumour was measured using a dial-gauge calliper (largest diameter × diameter at right angle). The groups differed not significantly regarding tumour size. Representative of three independent experiments, mean ± SD. (b) Immunohistochemically detection of CCL19 within the tumour of J558-CCL19-IgG2b-transfected cells documented intratumoral CCL19 expression in vitro. Indirect immunofluorescent staining of CCL19 with 10 μg/ml goat anti-mouse MIP-3β (R & D Systems, Wiesbaden, Germany) using peroxidase-conjugated mouse-anti-goat IgG (dianova) as secondary antibody and detection with tyramide signal amplification direct red (NEN Life Science Products, Boston, MA, USA), according to the manufacturer's instructions. Micrographs are representative of organs from three different recipient mice.