Table 1.

PCR primer sequences, product size and GenBank accession numbers for cytokine RT-PCR

Cytokine	PCR conditions	Primer sequence	5′/3′ bp position	GenBank accession no.	Product size (bp)
IL-2	15 s at 95°/1 min at 60·5°	5′-acc tct gcg gca tgt tct g	341–359/499–479	X01772	159
		3′-tcc acc aca gtt gct gac tca
IL-4	15 s at 95°/1 min at 60·5°	5′-tcg gca ttt tga acg agg tc	188–207/311–292	M25892	124
		3′-agc acc ttg gaa gcc cta c.
IL-5	15 s at 95°/1 min at 60°	5′-tgg aga ttc cca tga gca c.	105–124/194–174	X06270	90
		3′-gcc tca tcg tct cat tgc ttg
IFN-γ	20 s at 95°/20 s at 58°/1 min at 72°	5′-gc cac ggc aca gtc at	134–149/395–379	K00083	262
		3′-ttc gcc ttg ctg ttg ct
β-actin	20 s at 95°/20 s at58°/1 min at72°	5′-cgtgggccgccctaggcacca	24–44/209–193	M12481	186
		3′-acacgcagctcattgta