Figure 3.

Expression patterns of α NAC protein. Expression of α NAC protein in CD8⁺ cells cultured either with AF (a, b) or with anti-CD3 (c) At the indicated time points, cells were either FACS sorted into CD8⁺ CD57⁺ and CD8⁺ CD57^– cell populations (ai and bi) or harvested without sorting (c). Cell lysates were resolved on 10% SDS–PAGE. The membrane was probed with polyclonal anti-rabbit against α NAC protein. Densitometry scanning of α tubulin level in the FACS sorted CD8⁺ CD57⁺ and CD8⁺ CD57^– cell (aii and bii). Expression of α NAC protein was also investigated in T- and B-cell lines(d).