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. 2004 Jul;112(3):404–412. doi: 10.1111/j.1365-2567.2004.01901.x

Figure 1.

Figure 1

Pten+/– SHIP+/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice show an increased humoral response to the T-dependent antigen, nitrophenylacetyl (NP)-keyhole limpet haemocyanin (NP-KLH). Serum antibodies against NP-bovine serum albumin (NP-BSA) were assessed from six or seven mice of each genotype that had been challenged with NP-Ficoll or NP-KLH. Anti-NP titres [immunoglobulin M (IgM) and pan immunoglobulin G (IgG)] were determined by enzyme-linked immunosorbent assay (ELISA) on serially diluted serum samples. Bars indicate the average titre, and triangles represent individual mice. No differences were observed in either the IgM or IgG response to NP-Ficoll (a, b). However, statistically significant differences in IgM and IgG responses to NP-KLH were detected (c, d). *P < 0·05 compared with all genotypes, ***P < 0·005 compared with wild type and SHIP+/–, but not significant over Pten+/–. **P < 0·05 compared with wild type and SHIP+/–. WT, wild type; SH, SHIP+/–; PH, Pten+/–; DH, Pten+/– SHIP+/–.