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Reduced levels of Pten (phosphatase with tensin homology) and SHIP (SH2-containing inositol phosphatase) protein in Pten+/– SHIP+/– T cells. Whole-cell lysates from expanded CD4+ T cells of the different genotypes were examined for Pten and SHIP levels by immunoblotting. Numbers represent densitometric values obtained for Pten and SHIP protein bands from independent immunoblots, and the results are representative of lysates from two independent sets of littermate control mice.
