Figure 4.

In vitro generation of effector cells in the presence of anti-IFN-γ antibody. CD4⁺ memory effector T cells obtained from the peripheral blood of a healthy donor (normal) or from RASF (patient) were stimulated as described in Fig. 3 with immobilized anti-CD3 mAb (OKT3, 0.01 μg/ml), anti-CD28, and cytokines. In addition, cultures were carried out in the presence of increasing concentrations of a neutralizing anti-IFN-γ antibody as indicated. After priming, the cells were washed, rested for 2.5 days and restimulated with mitogen to assess intracellular cytokines; 10⁴ cells were collected for each analysis. The data are representative of at least two experiments. No significant difference was observed in the presence of anti-IFN-γ antibody (P > 0.05).