Skip to main content
. 2004 Jul;112(3):437–445. doi: 10.1111/j.1365-2567.2004.01895.x

Figure 3.

Figure 3

Effect of NF-κB inhibition on EBI3 and IL-12p35 mRNA expression. (a) HT-29 cells grown to confluence were treated with the proteasome inhibitor MG-132 (50 µm) for 30 min prior to stimulation with IL-1α or TNF-α (20 ng/ml) alone or in combination with IFN-γ (40 ng/ml) or left unstimulated (unstim.), followed by RT–PCR analysis for EBI3, IL-12p35, and β-actin mRNA. (b) HT-29 cells left uninfected, infected with control adenovirus (AdLacZ), or adenovirus expressing mutant IκBα as a superrepressor (AdSR) were left unstimulated or stimulated with TNF-α or IFN-γ, after which EBI3 and IL-12p35 mRNA expression was determined by real time PCR. Values are expressed as fold induction relative to cells that were not virus infected. Similar results were obtained in 3 repeated experiments.