Figure 5.

Effect of SP600125 on the phosphorylation of c-jun and MAPKAPK2. Western blot analysis of c-jun and phospho-c-jun (a) and p-MAPKAPK2 and β-actin expression (b) in lung tissue isolated from sensitized rats exposed to saline (saline) or to ovalbumin (ovalbumin) or to ovalbumin and pretreated with SP600125 (SP600125). Phospho-c-jun expression was observed in sensitized and ovalbumin-exposed rats and was inhibited in SP600125- treated rats. The p-MAPKAPK2 expression was increased in sensitized and ovalbumin-exposed rats and was not inhibited in SP600125-treated rats. β-actin was included as a control. A representative example of two out of three experiments for each group is shown (labelled 1 or 2). The mean density of bands from three rats in each group is shown on the right-hand side of the panel. *P < 0·05, **P < 0·01 as compared to group saline. #P < 0·05 as compared to group Ovalbumin. Data are shown as mean ± SEM.