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. 2004 Jul;112(3):489–495. doi: 10.1111/j.1365-2567.2004.01900.x

Figure 5.

Figure 5

Two-colour flow cytometric analysis of the expression of CD3, CD4 and CD8 on splenic lymphocytes from mice exposed to bisphenol A (BPA) during fetal life. Female mice were administered per os (p.o.) the indicated doses of BPA, daily over a period of 18 days, commencing on the day of pairing with males (day 0). On day 77, splenic lymphocytes from male (a, b, e, f) or female (c, d, g, h) littermates of 8 weeks exposed to control (a, c, e, g) and BPA (b, d, f, h) were stained with anti-mouse CD4, CD8, and CD3 monoclonal antibodies, as described in the Materials and methods.