Table 2.

Effect of neutralizing anti-type I interferon (IFN)-receptor and anti-interleukin-15 (anti-IL-15) immunoglobulin on the polyinosinic-polycytidylic acid [poly(I:C)]-induced activation of γδ T cells

	Medium	Anti-type I IFN-R	Anti-IL-15
Medium	11·9 ± 0·9	9·5 ± 1·1	12·3 ± 0·4
poly(I:C)	52·1 ± 9·9	18·8 ± 2·6	59·0 ± 2·4
IFN-α	58·8 ± 1·9	27·9 ± 2·2	–
IFN-β	40·9 ± 2·4	19·7 ± 5·8	–
IL-15	71·9 ± 2·7		18·3 ± 0·3

Primary peripheral blood mononuclear cells (PBMC) were cultured in medium alone, or in medium containing poly(I:C) (50 µg/ml) or the indicated cytokines (100 U/ml IFN-α, 100 U/ml IFN-β, 50 ng/ml IL-15), in the presence or absence of the following neutralizing antibodies: anti-type I IFN-receptor (anti IFN-α receptor chain 2; 5 µg/ml) or anti-IL-15 (5 µg/ml). After 24 hr of culture, the expression of CD69 on γδ T cells was assessed by two-colour fluorescence-activated cell sorter (FACS) analysis. Results represent the mean values ± standard deviation (SD), obtained in three separate experiments.