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Specificity of anti-human invariant chain 2C12 (a) and 5F6 (b) mouse monoclonal antibodies, determined by different antigen-immobilized ELISAs. 2C12 and 5F6 mAb were added in the concentration range of 1 ng/ml (9·4 × 10−12 m) to 5 µg/ml (3·4 × 10−8 m). Curve marked with (p41*) represents the signal of Alexa Fluor™ 488-labelled 2C12 mAb with recombinant inhibitory p41 Ii.
