Figure 3.

Lymph node distribution of p41 and p31 invariant chain isoforms (immunofluorescence). Human lymph node tissue sections were labelled with anti-Ii primary mAb (2C12 mAb and 5F6 mAb) and anti-mouse Alexa Fluor^™ 488-labelled secondary antibody in single labelling experiment. (a, b) 2C12 mAb(c) 5F6 mAb(d) 2C12 mAb, preblocked with inhibitory recombinant p41 Ii(e) control, without primary anti-Ii mAb and (F) 5F6 mAb, preblocked with non-inhibitory recombinant p31 Ii. The positions of sinus (arrows), paracortex (+) and cortex (*) are denoted. Bars: 50 µm.