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. 2004 Jul;112(3):471–480. doi: 10.1046/j.1365-2567.2004.01905.x

Figure 5.

Figure 5

RT–PCR result for IL-8 gene (300 bp product) mRNA expression at 48 hr. (a) Lane 1: 100 bp pair marker. Lane 2: Cytokine mixture stimulated A549 cells. Lane 3: IFN-γ stimulated A549 cells. Lane 4: IFN-γ + TNF-α stimulated A549 cells. Lane 5: TNF-α stimulated A549 cells. Lane 6: A549 cells infected with H37Rv. Lane 7: A549 cells infected with H37Rv and stimulated with IFN-γ. Lane 8: A549 cells infected with H37Rv and stimulated with cytokine mixture. Lane 9: A549 cells only. (b) β-actin gene mRNA (control, 687 bp product) demonstrating equal loading in all the corresponding lanes as in (a).

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