Table 2.
Correlation of nitric oxide production and mycobactericidal activity of A549 cells, infected with M. tuberculosis H37Rv and stimulated with various cytokines
| Treatment | 24 hr | 48 hr | |
|---|---|---|---|
| A549+H37Rv+IFN-γ | Nitric oxide(µmol/l) | 41·16±5·1 | 61·27±4·9 |
| CFU/ml/well (×104) | 9·285±0·29 | 6·858±0·37 | |
| % Reductionin CFU | 12·07% | 34·87% | |
| A549+H37Rv+(IFN-γ+TNF-α) | Nitric oxide(µmol/l) | 37·86±4·9 | 55·46±6·2 |
| CFU/ml/well(×104) | 9·083±0·64 | 6·7±0·54 | |
| % Reduction CFU | 13·74% | 36·37% | |
| A549+H37Rv +(IFN-γ+TNF-α+IL-1β) | Nitric oxide(µmol/l) | 48·86±4·9 | 56·75±7·7 |
| CFU/ml/well(×104) | 9·283±0·61 | 6·392±0·54 | |
| % Reductionin CFU | 11·84% | 39·29% | |
| A549+H37Rv | Nitric oxide(µmol/l) | 20·02±5·5 | 29·53±5·7 |
| CFU/ml/well(×104) | 10·26±0·2 | 10·62±0·48 |
Percent reduction was calculated from CFU/ml at 6 hr after amikacin treatment and washing in each set of experiments. CFU/ml at 6 hr was 10·53 ± 0·71 which was the basal value. Basal value of nitrite at 6 hr was 5· 68 ±3·1 µm/l. IFN-γ, TNF-αand IL-1β were added at a concentration of 250 units/ml, 10 ng/ml and 50 units/ml, respectively.