Figure 1.

Dectin-1 and TLR-2 and -6 colocalize in nascent zymosan phagosomes. V5-tagged TLR-2 and -6 (shown in red) and HA-tagged dectin-1 (green) colocalize within 5 min of initiating zymosan uptake. (a) Transmission image of two adjacent cells binding zymosan particles. (b) Dectin-1 HA staining. (c) TLR-2 V5 staining. (d) Staining showing colocalization. The cell indicated with an arrow was not transfected with TLR-2 and is presented asa staining control. (e) Transmission image of two adjacent RAW-D1 macrophages. (f) Dectin-1 is present in nascent phagosomes. (g) TLR-6 is also present in nascent phagosomes. (h) Merge shows that TLR-6 and dectin-1 colocalize. (Reproduced from ⁴⁵)