Figure 1.

Immunoglobulin-like transcript-2 (CD85j/ILT2) is highly expressed on antigen-specific cells in healthy and human immunodeficiency virus (HIV)-seropositive individuals. Representative flow cytometry analysis is shown of inhibitory natural killer cell receptor (iNKR) expression on total and cytomegalovirus (CMV)-specific CD8 T cells in peripheral blood mononuclear cells (PBMCs) from (a) a healthy HIV-seronegative individual and (b) an asymptomatic HIV-seropositive donor. The first dot-plot for each sample displays the percentage of human leucocyte antigen (HLA)-A0201-CMV tetramer-positive cells in the lymphocyte gate. All the tetramer-positive cells are CD8^high and form a well-separated population. Other dot-plots displaying NKR expression are gated on CD8^high cells. The percentage of tetramer-positive and total CD8⁺ cells that stain above background for each iNKR are shown. The high proportion of CMV tetramer-positive cells in (a) that are CD94⁺ is not representative of the group of normal donor samples (Table 2).