Figure 6.

Immunoglobulin-like transcript-2 (CD85j/ILT2) inhibits interferon-γ (IFN-γ) production by CD8 T cells. Non-adherent peripheral blood mononuclear cells (PBMCs) from one human immunodeficiency virus (HIV)-seropositive (a) and two HIV-seronegative (b–e) donors, preincubated with ILT2 blocking antibody, control antibody or no antibody, were mixed with Epstein–Barr virus (EBV) (b, c, e) or cytomegalovirus (CMV) (a, d) antigenic peptide-armed autologous adherent cells and analyzed for IFN-γ production. Percentages of CD8 T cells staining for intracellular IFN-γ are shown. The mean value ± standard deviation (SD) of the five experiments is also shown for each condition. The percentage of IFN-γ⁺ cells increases by approximately threefold when ILT2 engagement is blocked.