Table 1.
ISS-ODN modulation of Th2-biased adaptive immune profiles
| Intervention | %IgE increase | %IgG1 increase | IL-4 (pg/ml) | IL-5 (pg/ml) | IL-13 (pg/ml) | IFN-γ (pg/ml) |
|---|---|---|---|---|---|---|
| None | 478 ± 83 | 432 ± 119 | 2379 ± 840 | 2468 ± 783 | 146 140 ± 32 680 | 159 ± 23 |
| ISS-ODN (i.d.) | 187 ± 34* | 166 ± 44* | 472 ± 221* | 154 ± 60* | n.d.* | 772 ± 163* |
| C-ODN (i.d.) | 549 ± 186 | 798 ± 27 | 6086 ± 3039 | 2452 ± 1170 | 112 112 ± 21 493 | 173 ± 71 |
| ISS-ODN (i.n.) | 197 ± 47* | 134 ± 67* | 459 ± 137* | 186 ± 35* | 856 ± 856* | 617 ± 131* |
| C-ODN (i.n.) | 425 ± 101 | 413 ± 57 | 2517 ± 704 | 2459 ± 619 | 105 151 ± 5704 | 133 ± 37 |
| DXM | 200 ± 37* | 286 ± 175 | 2782 ± 1196 | 902 ± 86 | 197 433 ± 81 995 | 164 ± 41 |
Mice (four mice/group) were sensitized and challenged as outlined in Fig. 1. Sera were collected for OVA-specific IgE and IgG1 determinations 1 day before the first and 1 day after the last i.n. OVA challenges. (Post-challenge immunoglobulin/pre-challenge immunoglobulin) × 100=% immunoglobulin increase. Splenocytes were harvested to assess OVA specific cytokine production at the time of the second bleed. Results are reflective of three separate experiments.
n.d.=none detected.
*
P ≤ 0.05 for comparisons to OVA sensitized and challenged mice receiving no intervention.