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The serum IFN-γ, IL-4 and ALT levels after α-GalCer administration in young (6 weeks) and middle-aged (25 weeks) mice pretreated with IL-12 or PBS. Young and middle-aged mice pretreated with IL-12 or PBS 24 hr before were administered with α-GalCer intravenously and peripheral blood samples from five individual mice were collected at the indicated time points from the retro-orbital plexus. The data are expressed as the mean ± SE from five mice of each group.
