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. 2004 Sep;113(1):35–43. doi: 10.1111/j.1365-2567.2004.01932.x

Figure 3.

Figure 3

(a) In vitroα-GalCer-stimulated IFN-γ and IL-4 production from liver MNC of mice pretreated with IL-12 or PBS. Liver MNC were obtained from young or middle-aged mice pretreated 24 hr before with IL-12 or PBS were stimulated with α-GalCer in vitro for 24 hr and culture supernatants were subjected to ELISA. (b) In vitroα-GalCer-stimulated IFN-γ and IL-4 production from liver MNC pretreated with IL-12 (25 ng/ml). Liver MNC were obtained from young or middle-aged mice and cultured with medium supplemented with IL-12 (in 5 μl PBS, final concentration; 25 ng/ml)or 5 μl PBS for 24 hr and thereafter were stimulated with α-GalCer in vitro for 24 hr and culture supernatants were subjected to ELISA. The data are expressed as the mean ± SE from four mice of each group.