Figure 4.

Isolation of active components of lipid-associated membrane proteins (LAMPs). Mycoplasma penetrans (a) and M. fermentans (b) LAMPS were dissolved in 6 m guanidine hydrochloride, and 100 µg of LAMPs was separated by reverse-phase high-performance liquid chromatography (HPLC). Elution was performed using a 0–100% linear gradient of water/2-propanol. Each fraction was added to THP-1 cells transfected with 0·1 µg/ml pFLAG-TLR2 and 0·1 µg/ml pNF-κB-luc. These results are representative examples of the three independent experiments.