Toll-like receptor (TLR) usage of Mycoplasma penetrans lipid-associated membrane proteins (LPMp) and M. fermentans lipid-associated membrane proteins (LPMf). 293T cells transfected with 0·01 µg/ml pFLAG-TLR2, 0·01 µg/ml pNF-κB-luc, and 0·2 µg/ml pFLAG-dTLR1 or pFLAG-dTLR6, were stimulated with 10 ng/ml LPMp, 10 ng/ml LPMf, 1 nm MALP-2, and 1 µg of Pam3CSK4 (P3CSK). All values represent the mean and standard deviation (SD) of three assays. DN, dominant negative.