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Lipoprotein lipase (LPL) treatment of Mycoplasma penetrans lipid-associated membrane proteins (LPMp) and M. fermentans lipid-associated membrane proteins (LPMf). One microgram of LPMp and LPMf was treated with 100 µg/ml LPL at 37° for 2 hr. 293T cells transfected with 0·01 µg/ml pNF-κB-luc and 0·01 µg/ml pFLAG-TLR2 were stimulated with 10 ng/ml LPL-treated LPMp and LPMf. All values represent the mean and standard deviation (SD) of three assays. PBS, phosphate-buffered saline.
